HRP Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab199560) (2024)

Overview

• Product name

  HRP Anti-APG5L/ATG5 antibody [EPR1755(2)]
  See all APG5L/ATG5 primary antibodies

• Description

  HRP Rabbit monoclonal [EPR1755(2)] to APG5L/ATG5

• Host species

  Rabbit

• Conjugation

  HRP

• Tested applications

  Suitable for: WBmore details

• Species reactivity

  Reacts with: Mouse, Rat, Human
  

• Immunogen

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• Positive control

  • WB: Raji, Raw264.7, NIH3T3 andPC-12 whole cell lysates.

• General notes

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free production

  For more information see here.

  See Also

  Arcus Infrastructure Partners and APG Asset Management Acquire TDFAPi Group Corp (NYSE:APG)Oldcastle APG hiring Yard Foreman - Phoenix, AZ in Phoenix, AZ | LinkedInOldcastle APG hiring Forklift Operator / Yard - Cowpens, SC in Cowpens, SC | LinkedIn

  Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

• Form

  Liquid

• Storage instructions

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

• Storage buffer

  pH: 7.40
  Preservative: 0.1% Proclin 300 Solution
  Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS

• Concentration information loading...

• Purity

  Protein A purified

• Clonality

  Monoclonal

• Clone number

  EPR1755(2)

• Isotype

  IgG

• Research areas

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Associated Proteins

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ub-like Proteins

  • Cell Biology
  • Apoptosis
  • Phagocytosis

  • Cardiovascular
  • Heart
  • Autophagy
  • APG gene products

  • Cancer
  • Signal transduction
  • Autophagy

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Autophagy and mitophagy

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Autophagy and mitophagy
  • APG gene products

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Cell Death
  • Apoptosis
  • Phagocytosis

  • Cancer
  • Cell Death
  • Apoptosis
  • Metabolism

  • Cancer
  • Cell Death
  • Autophagy
  • Signal Transduction

  • Cancer
  • Cell Death
  • Autophagy
  • APG gene products

  • Neuroscience
  • Processes

Associated products

• Alternative Versions

  • Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327)
  • Anti-APG5L/ATG5 antibody [EPR1755(2)] - BSA and Azide free (ab221604)

• Isotype control

  • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)

• Recombinant Protein

  • Recombinant Human APG5L/ATG5 protein (ab103787)

• Related Products

  • ECL Substrate Kit (High Sensitivity) (ab133406)

Applications

Target

• Function

  Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
  May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.

• Tissue specificity

  Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.

• Sequence similarities

  Belongs to the ATG5 family.

• Post-translational
  modifications

  Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
  Acetylated by EP300.

  See Also

  Jarltech Homepage | Jarltech.com

• Cellular localization

  Cytoplasm. Preautophagosomal structure membrane. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.

• Information by UniProt

• Database links

  • Entrez Gene: 9474 Human
  • Entrez Gene: 11793 Mouse
  • Entrez Gene: 365601 Rat
  • Omim: 604261 Human
  • SwissProt: Q9H1Y0 Human
  • SwissProt: Q99J83 Mouse
  • SwissProt: Q3MQ06 Rat
  • Unigene: 486063 Human

  see all

• Alternative names

  • APG 5 antibody
  • APG 5L antibody
  • APG5 antibody

  see all

Images

Protocols

Datasheets and documents

References (1)